figure4.png
ja1c13568_0005_edited.jpg

Welcome to the John Burke Lab at the University of Victoria

We are interested in studying the molecular basis for how enzymes that modify lipid phosphoinositides and the Ras superfamily of G-proteins are regulated. These enzymes mediate many important cellular functions including growth, membrane trafficking, cytokinesis, and proliferation. Due to the key roles that these enzymes play, they are frequently involved in many human diseases. The main goal of our research is to define the mechanism of how these enzymes are involved in disease, and design novel therapeutic strategies for treating disease.

We address this problem using an innovative mixture of biophysical tools, primarily focusing on structural mass spectrometry and X-ray crystallography.

We combine X-ray crystallography, electron microscopy, HDX-MS, and biophysics  to characterise the structure, regulation and interactions of membrane signalling complexes and their roles in human disease